Closed:Alternate Splicing using Limma

Question

[thal.kan49]

Hello, I'm trying to follow de Limma package to analyse some RNA-Seq data. The workflow works clearly but I've some problems with the alternate splicing:

> diffSplice(fit, geneid = "GeneID", exonid = "Start")
Total number of exons:  16772 
Total number of genes:  16772 
Number of genes with 1 exon:  16772 
Mean number of exons in a gene:  1 
Max number of exons in a gene:  1 
Error in diffSplicefit.de, geneid = "GeneID", exonid = "Start") : 
  No genes with more than one exon

I don't find where is the problem, may someone help me to fix this problem if possible. Best regards.