Question: GEO differential expression analysis
0
gravatar for yehchan57
11 months ago by
yehchan5710
China
yehchan5710 wrote:

ID translation and remove duplicated genes which different probes are mapped are routine procedure in differential expression analysis using GEO series. However, when I do differential expression first then translate ID and remove duplicated genes, the number of DEG exceeds the former. Why?

deg R geo • 348 views
ADD COMMENTlink modified 11 months ago by zephyr_falcon80 • written 11 months ago by yehchan5710
1
gravatar for zephyr_falcon
11 months ago by
New York, USA
zephyr_falcon80 wrote:

That's probably because of multiple testing. If you perform DE analysis BEFORE removing duplicates, the number of genes used in DE analysis is larger than that of DE analysis AFTER removing duplicates, and it will end up with lower FDRs because there are more genes. And lower FDR can be leaded to more DE genes.

ADD COMMENTlink written 11 months ago by zephyr_falcon80
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