question about signal of IP / Input
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4.8 years ago
ch8316f5eyu ▴ 10

I wonder why the log2(IP/Input) value of the distal region is not about 0. For example:enter image description here

The code used to generate the bamcompare file is:

bamCompare --scaleFactorsMethod readCount --minMappingQuality 1 --samFlagExclude 256 --operation log2 --outFileFormat bigwig --binSize 20 --pseudocount 5 -b1 Input.bw -o IP.bw

I think the possible reason is: There are too many reads in some unknown region in Input sample. Is this explanation reasonable? Or there are other reasons?

ChIP-Seq • 885 views
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