Question: Normal sample variant calling method without PON ?
0
gravatar for Igor Islanov
16 months ago by
Russia, Moscow, Petrovsky Russian Research Center of Surgery
Igor Islanov0 wrote:

Good day,

I'm trying to recreate pipeline that was described in Bioinformatics Analysis of Whole Exome Sequencing Data and i'm facing some problems. Searching for answers i came across various posts, but almost everything is related to either with tumor variants or with Panel of Normals (which i don't have as pipeline only about to be used in the future and only 1 sample of WES available right now).

Is there a way to do variant calling with normal sample without PON ? I'm trying to use Mutect2 as the papers and posts suggest, but encountering various problems and errors, mostly related to interval list and/or lack of tumor/PON samples.

sequencing mutect2 wes gatk • 334 views
ADD COMMENTlink modified 16 months ago • written 16 months ago by Igor Islanov0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1516 users visited in the last hour
_