Categorizing prokka outputs

Question

[biobiu]

I've annotated assembled bins using prokka. Now I want to cluster the genes by function, pathways etc. I thought to categorize enzymes by ec number. Any recommendations on how to do that for non-enzymatic protein coding genes?

[Mark]

You could blast those genes to find a homolog with an EC number and use that in your analysis. You could also use gene ontology (GO) or clusters of orthologous (COG) analysis to cluster genes/proteins into functions.