I am trying to run primersearch to get a large batch of primer pairs against a directory of around 45 fasta files. When I try to run it for one using the command below, I get the error that follows. I also have the debug file, but I don't know how to upload that. I've tried with EMBOSS/6.5.7 and EMBOSS/6.4.0. Has anyone seen this error, or at least have a clue what it means?
primersearch -seqall sequences/SAMN04014954.fasta -infile primerList.txt -mismatchpercent 10 -outfile SAMN04014954 Search DNA sequences for matches with primer pairs Warning: Illegal character '/' EMBOSS An error in primersearch.c at line 499: Illegal pattern
Thank you for your help.