Question: appending information to seurat object meta.data
1
cook.675 • 60 wrote:
I have a seurat object called AllCells.combined, and I wish to identify within the object all the cells that have expression of gene "Ighm" > 0; and also identify all cells that have expression of "Ighm" = 0
An easy way to do this would be to append it to the meta.data but I can't quite get it.
I tried:
AllCells.combined$IGHMPOS <- WhichCells(AllCells.combined, expression = Ighm > 0)
and got this error: Error: Cannot add more or fewer cell meta.data information without values being named with cell names
I then tried this:
AllCells.combined$IGHMPOS <- AllCells.combined["Ighm" > 0, ]
and got this error:
Error in [.Seurat(AllCells.combined, "Ighm" > 0, )
Incorrect number of logical values provided to subset features`
I know its something simple Im missing
Thanks in advance!
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modified 8 months ago
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atakanekiz • 260
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written
15 months ago by
cook.675 • 60