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4.4 years ago
karunaworah
•
0
Can any one help me for a R script to break the protein sequences by enzymatic action.(trypsin). If trypsin breaks protein sequences at K and R, How can i get all the peptide fragments after cutting. K and R for a sequence. Input file is in Fasta format
Input File
sp|Q13515|BFSP2_CON-HUMAN Phakinin OS=Homo sapiens GN=BFSP2 PE=1 SV=1 MSERRVVVDLPTSASSSMPLQRRRASFRGPRSSSSLESPPASRTNAMSGLVRAPGVYVGT
sp|P15924|DESP_CON-HUMAN Desmoplakin OS=Homo sapiens GN=DSP PE=1 SV=3 MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTG TMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEI
Output like: All peptide between of length minimum 10 and max 20 sequnces.
GN=BFSP2
RVVVDLPTSASSSMPLQ RASFRGPRSSSSLESPPASRTNAMSGL KAPGVYVGT
