I have prepared 4 libraries using a custom Ampliseq for Illumina panel. Despite that the libraries have the expected size based on Agilent 2200 tapestation run, their quantities are really low ranging from 0.123 ng/ul -0.266 ng/ul (Qubit HS DNA assay) / 247pM-1nM (NEBNext® Library Quant Kit for Illumina). Do you have any idea on how to increase the libraries' concentrations?
Question: Why do I get Ampliseq for Illumina libraries of low concentration?
14 months ago by
stefaniab • 0
stefaniab • 0 wrote:
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