Mega X help - phylogenetic tree

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4.5 years ago

SILE1_17 • 0

Hi,

I'm VERY new to bioinformatics, and for my uni course I'm doing a project analysing the geographic spread of chytrid fungus. I have used NCBI to identify Batrachochytrium dendrobatidis (chytrid fungus) ITS1 & ITS2 partial sequences, I have saved these in a FASTA file and uploaded them to Mega x to produce a phylogenetic tree. However my out-groups are only out when I use UPGMA, the bootstrap values are mostly very bad and the tree doesn't look right at all, so I think I must be doing something wrong or maybe my sequences are bad. Just looking for some help and advice.

Thank you!!!

Mega X Phylogeneic Tree Maximum Likelihood UPGMA • 2.0k views

ADD COMMENT • link 4.5 years ago by SILE1_17 • 0

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Is it possible to provide a picture of the tree? Did you look at the genetic differentiation of the sequences between samples? Would you expect good bootstrap values or a good resolution of the tree based on the genetic differentiation of the samples at all?

ADD REPLY • link 4.5 years ago by solanum ▴ 10

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