Data set containing corresponding RNA-seq and RT-Qpcr data
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4.4 years ago
chtrgswm ▴ 20

Can anybody please direct me to a study where they have published RNA seq and RT-qPCR data of the same organism? There are experiments that have validated RNA-seq using RT-qPCR, but they published only fold changes and not the raw Ct values. My work requires both the raw read counts and raw ct values. The disease is irrelevant.

RNA-Seq RT-qpcr sequencing • 1.0k views
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There is this quite old comparison of the MAQC data which is regularly repeated (e.g. in the ABRF study), but the original paper contained assays like TaqMan, QuantiGene, and StaRT-PCR:

https://www.ncbi.nlm.nih.gov/pubmed/16964229

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