I am doing some experiment using BowTie which is a short read aligner that aligns short DNA sequences (reads) to the human genome. Now, I want, to experiment with it on 150 bps reads. So, I want DNA sequencers 150 bps output file. For example, output of this (Genome Analyzer IIx). Can anybody please help me, where can I find it ? Or is there any BioInfo community who can help me about this ?
No. I want format like http://en.wikipedia.org/wiki/FASTQ_format (see Encoding section, at the last of the page) but for 150 bps.
The Short Read Archive contains FASTQ data. They have a conversion script to dump SRA to FASTQ.
Thanks a lot. But, I really don't find any folder saying that they contains, 150 bps length Short Read.
see changes to main post above.