Calculating in SEURAT, list of highly dysregulated genes across conditions in a particular cluster.
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11 months ago
singcell • 0

I am trying to integrate two datasets from different conditions using Seurat vignette "Integrating stimulated vs. control PBMC datasets" #3.1 I wanted to Identify highly dys-regulated genes or highlighting genes that exhibit dramatic responses across conditions in a particular cluster.

For example, in the scatter plots below dots further away from line are dysregulated (exhibit dramatic responses) but I need to supply the list of genes to label those outlier point (genes.to.label).

My question is, Is there any way to label those outlier DOTS without knowing identity of those dots beforehand or to determine the list of genes belonging to a cluster that are exhibiting dramatic responses between two treatment conditions.

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rna-seq seurat Stimulated vs Control PBMCs • 314 views
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