Hi, I have been working on the enrichment analysis on mouse RNA-seq data (mm10) I have been trying to upload my expressed gene file (about 15,000 rows are included. 120KB) as background, but unfortunately, I am unable to upload it on DAVID, since the website doesn't respond.
I have two files: up-regulated gene list and down-regulated gene list for the enrichment analysis and I want to create functional annotation chart per file.
Do you know how to upload my background file on DAVID successfully? Does an uploading error happens a lot in DAVID? I am also looking for any other tools for enrichment analysis, what would you recommend?
I looked at "Enrichr," but it seems like I cannot upload my own background file on the website. But, I need to upload a customized background file, so please let me know if there is any tool that I can use my background file like in DAVID.
I am new to bioinformatics, so any type of assistance is greatly appreciated. Thank you.