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4.0 years ago
ML
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20
I have 20 RNAseq samples control and experimental. Now, We have planned to perform Co-expression analysis and found WGCNA an R package is the most used package for the same. I tried the test data and it works well. I am confused regarding the preparation of input file. We ran Quality check than trimmomatic tool and mapped with Hisat2 and used featurecount and to find degs from the 20 samples we used edgeR. We obtained upregulated and downregulated degs but how to make input file for WGCNA. We are clueless and new to this domain. Please guide us to what I am doing wrong and how to make expression table in csv for 20 samples. This is the first post so please forgive me if I am wrong or my english seems to be poor. Thankyou
