Alternative tool for split'N'trim

Question

[aiswaryabioinfo]

I am trying to identify SNPs from RNA-Seq data. Is there any alternative tool for split'N'trim in usegalaxy server

[aiswaryabioinfo]

Sir, I dont have much computational facility to run RNA-seq variant calling in computer so I am relaying onlie platforms like galaxy. In order to preprocess the aligned BAM file I marked the duplicates with Picard. now for splitting I am not getting any online tools. So Can anyone of you help me out.