I'm converting a
SingleCellExperiment object to a
Seurat object, and I'd like to be able to regress out the effect of % mitochondrial DNA when running
SCTransform for normalization & detection of highly variable genes. However, running
as.Seurat does not add the necessary
nFeature_RNA metadata to the new
Seurat object. Is there a non-exported function in
Seurat that I could use to add this information?