I have a count matrix of single cell data with 122 cells. I am trying to process it with the standard Seurat workflow, however, the UMAP reduction is yielding strange results. Basically, no clusters are forming. I'm wondering if ~100 cells is too low for UMAP. Does anyone know what might be going on here? I can provide my code if necessary
Edit: I found if I raise the "resolution" argument in the "FindClusters" function then clusters are picked up. I'll have to read more about what the "resolution" means in this context.