Finding the centromere and telomere of cattle genome (Bos Taurus)

Question

[Frieda]

Does anyone know how I can get the coordinates of the centromere and telomere of a chromosome of reference cattle(Bos Taurus) genome using online tools like NCBI?

I have searched a lot, but I could not find anything useful. Based on my findings, wet lab analysis is required to locate the centromere and telomere of a chromosome, but I believe that the coordinates of these regions should be avaliable for the reference genome.

Answer 1

[antonioggsousa]

Hi,

I know that telomeres, and I believe that also centromeres, are chomossomic regions quite difficult/impossible to assemble because they're comprised of regions of long-stretches of repetitive DNA. So, you probably will not find them, or even if you find them they will be quite incomplete or you cannot trust on those contigs/scaffolds. Though with long-read sequencing, e.g., PacBio, some of these regions can be at some extent solved.

I hope this helps,

António