Hi, I was trying Jellyfish on some downloaded data: Illumina-Ecoli100PE and the data sequenced in our lab. But none of them showed the curve like the first link do. All of mine results were the same: just downward, no peak (the 1-multiplicity had the most k-mers), no matter what K-mer used (tried 13,17,21,27,31). And because the current version supports fasta and fastq format, so I used this E.coli example directly as input file.
I believed results like this won't do any help to my purpose (find the peak, calc the coverage, and finally estimate the genome size) .
So, any of you have used this tool before? Could you share some exp? Maybe this tool just work normally for some certain kind of seq data? Thanks for your kindly help :)