hello, according to wiki answers this is the definition of + /- strand :
The positive strand of DNA is the one whose sequence contains the instructions for building a protein. The negative strand merely contains the complementary sequence, according to the base-pairing rules (A pairs with T, C pairs with G); the negative strand is not normally transcribed into RNA nor translated into protein.
I've got a .bed file that contains the following information:
**start end gene strand** NC_011191 3829 3899 trnI(gat) + NC_011191 3898 3969 trnQ(ttg) -
for the first line there is a + beneath strand column, and the second line has - . I want to extract the genes' sequence from the fasta file for nc_011191. according to the above definition i got confused : shall I for the second gene (-) take the complementary of the sequence in fasta file ? or something else ? thank you in advance