Entering edit mode
3.7 years ago
robinycfang
▴
20
Hi,
So I developed an algorithm to quantify some transcripts. I want to do differential expression analysis on these transcripts (control vs treatment). Initially, I calculated log2FC and did Wilcoxon ranked test on it, and found a significantly differentially expressed transcript. Then, I did Wilcoxon directly on control vs treatment, but for the same transcript, I found no difference. So I am wondering, when we do DE analysis, what's the null hypothesis? Is it log2FC = 0 or ctrl = tret? The two null hypotheses look literally the same, but why we get different results eventually?
You need to provide more information on your experiment. Right now it's unclear what your methodology is, and what question you are trying to address.