I am doing a differential expression analysis on miRSeq data. Since the reference miRNA sequence was not available in miRBase, I need to download them from a different database, PmiREN. I am confused on which miRNA reference sequences are to be downloaded to perform mapping using mirDeep2. Here is the list of reference sequences available:
Mature_miRNA_expression Mature_miRNA_seqequence miRNA_stem-loop_expression miRNA_stem-loop_secondary_structure miRNA_stem-loop_sequence miRNA_stem-loop_with_20bp_flanking_secondary_sequence miRNA_stem-loop_with_20bp_flanking_secondary_structure Star_miRNA_expression Star_miRNA_sequence Syntenic_block_info
Can anyone please guide me on this? Great thanks in advance!