Reference sequence for miRNA-Seq differential expression analysis
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13 months ago
fawazfebin ▴ 100

Hi

I am doing a differential expression analysis on miRSeq data. Since the reference miRNA sequence was not available in miRBase, I need to download them from a different database, PmiREN. I am confused on which miRNA reference sequences are to be downloaded to perform mapping using mirDeep2. Here is the list of reference sequences available:

Mature_miRNA_expression
Mature_miRNA_seqequence
miRNA_stem-loop_expression
miRNA_stem-loop_secondary_structure
miRNA_stem-loop_sequence
miRNA_stem-loop_with_20bp_flanking_secondary_sequence
miRNA_stem-loop_with_20bp_flanking_secondary_structure
Star_miRNA_expression
Star_miRNA_sequence
Syntenic_block_info

Can anyone please guide me on this? Great thanks in advance!

miRNA-Seq differential expression miRDeep2 • 263 views
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