Question: (Closed) Why is the DNA concentration and absorbance ratios fluctuating?
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gravatar for sidolfus94
4 months ago by
sidolfus940
sidolfus940 wrote:

I have done DNA extraction on human saliva using Promega ReliaPrep™ Blood gDNA Miniprep System that have been expired for more than five years now. When I initially checked the DNA concentration and the absorbance ratio, it shows a decent DNA concentration (~100 ng/ul) and decent absorbance ratios (A260/A280 = ~1.8 and A260/A230 = ~2.2). When I checked the DNA concentration and absorbance ratio a second time sometimes within minutes to days from the first check, the DNA concentration and absorbance ratio either drops or increases wildly.

For every nanodrop and qubit measurements, I have vortexed the tube first. For nanodrop specifically, I have used 2 µL and make sure to eliminate air bubbles.

Why is there many fluctuations in DNA concentration and absorbance ratios on my salivary human DNA samples?

Apologies if this is the wrong place to ask.

genome • 115 views
ADD COMMENTlink written 4 months ago by sidolfus940

Hello sidolfus94!

We believe that this post does not fit the main topic of this site.

This is not bioinformatics-related. Please ask e.g. at SeqAnswers, ResearchGate or a suitable SubReddit.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 4 months ago by ATpoint44k
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