Vst estimation across different populations from copy number data

Question

[rthapa]

Hi, I want to estimate Vst value across different population based on the copy number data. I have 5 populations and in each population, I have 10 samples. I wonder in order to estimate Vst values, do I need to average the CN values across samples from each population and then compare across the population? Is there any way to estimate Vst across population from copy number data of all samples without calculating average value? I would appreciate any suggestion. Thanks