Translation for genes with introns
0
0
Entering edit mode
12 months ago
DNAlias ▴ 30

I used tBLASTn to find a homolog for a human RefSeq gene in an unannotated genome. I want to find the corresponding protein sequence, but I'm not sure what is the best way to do this for Eukaryotic genes because they can have introns. Is there a particular program or method that I can use without annotating the whole genome?

Thank you

translation eukaryote • 255 views
ADD COMMENT
0
Entering edit mode

My instinct is to back translate and then to align the two nucleotide sequences, with manual adjustments if there are any small indels, cut out large gaps representing potential introns, translate the resulting sequence, and align the new protein sequence to the original protein sequence for the query to verify. Please let me know if there is a better or more streamlined way to do this.

ADD REPLY

Login before adding your answer.

Traffic: 2452 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6