Question: How to deal with single nucleus data in Seurat
gravatar for bellymountain
5 weeks ago by
bellymountain0 wrote:

Dear all, I am by no means a bioinformatician, but because of the pandemic I have been looking for parts of my work I can do from home, so please forgive me if I ask extremely basic questions.

I obtained a data set of single nuclei from the Drosophila antenna, and my boss suggested using the Seurat pipeline to analyse the data. I did the Seurat tutorials but none of them are specific for nuclei.

Does anyone have experience how to deal with this kind of data or can point me into the direction of some more information? For example in the QC step mitochondrial genes are excluded but my nuclei data should not have any of those to begin with.

Thank you for your advice, any help is appreciated.

ADD COMMENTlink written 5 weeks ago by bellymountain0

You can proceed as normal with single nuclei data.

ADD REPLYlink written 5 weeks ago by rpolicastro2.3k
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