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                    5.0 years ago
        Kumar
        
    
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    170
    Hi, I have 187 samples with two factors: (affected and unaffected) and (male and female) for RNA-Seq data analysis. I am using DESeq2 for DGE analysis. Therefore, I am trying to make a PCA plot to make different groups in three dimensional.
I have tried to make a PCA here 
. However, I need to make it in three dimensional with different groups.
Please suggest if I can make a PCA plot for this study. 
This has been discussed several times on Biostars and elsewhere. Several answers here that will be helpful for you.