Entering edit mode
3.5 years ago
chimerajit
•
0
I am using 3 extra sequence with GRCh38 as my sample have some vector with gene in it for indexing I did
hisat2-build -p 16 seq1.fa, seq2.fa,GRCh38.fa Genome
my question is if I insert new sequence in reference and align, then how I will annotate the same when I am using standard GTF file?
How Can I update my GTF file or what is the approach for annotation?
