hisat2-build multiple fasta and Annotation
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12 months ago
chimerajit • 0

I am using 3 extra sequence with GRCh38 as my sample have some vector with gene in it for indexing I did

hisat2-build -p 16 seq1.fa, seq2.fa,GRCh38.fa Genome

my question is if I insert new sequence in reference and align, then how I will annotate the same when I am using standard GTF file?

How Can I update my GTF file or what is the approach for annotation?

RNA-Seq Annotation GTF • 350 views