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4.9 years ago
sara.behrozi7
•
0
does STRT-seq protocol has any barcode file?
AATGATACGGCGACCACCGATNNNNNNGGGXX..XXCTGTCTCTTATACACATCTGACGCXXXXXXXXTCGTATGCCGTCTTCTGCTTG i.e (21bp of Sequence) + (6bp UMI) + (Variable bp of Sequence again) + "GACGC" + (8bp Cell Barcode) + (Variable bp of Sequence again)
this is my barcode format, how should I write barcode pattern for UMI tool extract?
