Question: Sequence length distribution after adapter trimming using trimmomatic
gravatar for GK1610
12 days ago by
United States
GK161090 wrote:

I have a chipseq library with sequence distribution of mean 150 from fastqc. After adaptor trimming, the distribution became from 35-149. quite wide.

I am getting acceptable NSC but RSC < 0.1, quality tag < 0 from Phantompeakqualtools tools. My guess is it is coming from varying fragment distribution.

Is there any way I can save this library?

alignment • 77 views
ADD COMMENTlink modified 11 days ago • written 12 days ago by GK161090
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