P3in file for primer3 : 0 records created

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3.2 years ago

avnim ▴ 10

I am trying to design ssr primer using published genome. My .misa file was created without any error with 4090 ssr but I am getting 0 records created in p3in file. I have removed spaces from the header and even tried by converting fasta file to single line fasta. Still either it shows 0 records created or file gets prepared without sequence template. I have tried different suggestions provided in previous threads related to same but still got no result. Please help

sequence misa primer3 • 1.0k views

ADD COMMENT • link 3.2 years ago by avnim ▴ 10

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Which versions of Primer3 and MISA are you using?I think MISA needs Primer3 1.1.3, newer versions slightly changed the output format and broke MISA's parsing.

ADD REPLY • link 3.2 years ago by h.mon 35k

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After your suggestion, I tried using the lower version of Primer3 also but still not able to generate primers.

ADD REPLY • link 3.2 years ago by avnim ▴ 10

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thank you for your suggestion h.mon.

ADD REPLY • link 3.2 years ago by avnim ▴ 10

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