kallisto preprocessing-Error: unspliced. bus has no BUS records

Question

[gabriella.wangyi1986]

Hi guys,

I'm trying to pre-process my fastq file to get loom files. I'm working on mouse models, so I followed kallisto to build index for ref, I split the index into 8 parts (-n 8). When I ran pre-processing, I put all the index files on the command but seems it's not working, could you please help me with the issue?

Thank you in advance!

Yi

!kb count --loom -i index.idx_intron.6,index.idx_intron.5,index.idx_intron.4,index.idx_intron.3,index.idx_intron.2,index.idx_intron.1,index.idx_intron.0,index.idx_cdna -g t2g.txt -x 10xv3 -o AIRE_GW_Het_fastqs \
-c1 cdna_t2c.txt -c2 intron_t2c.txt --workflow lamanno --filter bustools \
Het_S27_L004_R1_001.fastq.gz \
Het_S27_L004_R2_001.fastq.gz 
kb_python.validate.FileVerificationFailed: Het_fastqs/tmp/unspliced.bus has no BUS records