how to annotate Circular RNA gene type?
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7 months ago
modarzi ▴ 140

Hi, I detected Circular RNA through RNA-seq via CIRI2 as a Circular RNA identifier tool.

E.g., for one sample, I got the below result:

 circRNA_ID   chr circRNA_start circRNA_end #junction_reads SM_MS_SMS   #non_junction_reads junction_reads_ratio   circRNA_type     gene_id   strand    junction_reads_ID                                                                                                                                                                          

chr20:30954187|30956926 chr20   30954187 30956926  3    1_3_0              4                  0.600                intergenic_region    n/a      +       SRR1427487.35782013.1

Now, I need to understand annotation Circular RNA. How can I annotate Circular RNA?

I appreciated if you share your comment with me.

RNA-Seq Circular RNA annotation • 231 views
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try using CIRIquant, you can provide the outputs from CIRI2.

Looks like a ciRNA with no parent gene though from your output..

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Thank you for your comment. I have a question about your second sentence. do you mean my circular RNA record belongs to a region that its gene name has not been annotated?

I appreciated if you share your comment with me.

Best Regards,

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No no no.. sorry I phrased that poorly. It is from an intergenic region!

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