DiffBind with duplicates

Question

[jc2842]

Hi I'm a novice for ChIP-seq and trying to use DiffBind.

I have ChIP-seq dat of WT and mutant1 for H3K27me3. Each genotype has 2 replicates.

When I try the differential analysis, I get an error message "2 groups and at least triplicates for each group is required".

I understand triplicates are kinda minimum with meaningful stats but as a preview I wonder if I can run with duplicates before I generate more replicates.

Answer 1

[jc2842]

got it. i need to use minMember option. thanks.