Question: Incompatible Contigs Issue In Mitochondria Dna
gravatar for ckr123
6.1 years ago by
ckr12310 wrote:


I am a newbie working on mitochondria DNA sequencing. One error message I had during GATK quality score recalibration was " incompatible contigs Input files reads and reference have incompatible contigs: No overlapping contigs found.". My SAM files were aligned to the rCRS reference (gi|251831106|ref|NC_012920.1|), but the known SNP vcf files from NCBI FTP has contig MT. I tried to replace gi|251831106|ref|NC_012920.1| with MT in my SAM and reference files, but the same error message remained. Any ideas?


index the reference FASTA

tmap index -f ~/refseq/RCRS.fa samtools faidx ~/refseq/RCRS.fa

Default TMAP alignment

tmap mapall -f ~/refseq/RCRS.fa -r ~/ion_torrent/fastq/IonXpress_002_XXX.fastq -v stage1 map1 map2 map3 > ~/ion_torrent/map123/0403/SAM/sample1_normal.sam

Convert SAM to BAM

samtools view -bS ~/ion_torrent/0403/map123/SAM/sample1_normal.sam > ~/ion_torrent/0403/map123/BAM/sample1_normal.bam

Sort and index BAM file

samtools sort ~/ion_torrent/0403/map123/BAM/sample1_normal.bam ~/ion_torrent/0403/map123/BAM/sample1_normal.sorted.bam samtools index ~/ion_torrent/0403/map123/BAM/sample1_normal.sorted.bam

java -Xmx8000m\=tmp -jar /bin/GenomeAnalysisTK-2.4-9-g532efad/GenomeAnalysisTK.jar -T BaseRecalibrator -R ~/refseq/RCRS.fa -I ~/ion_torrent/0403/map123/BAM/sample1_normal.sorted.bam -knownSites ~/refvcf/MT-1409-CEU.vcf -o ~/ion_torrent/0403/map123/GRP/sample1_normal.recal.grp -plots sample1_normal.recal.grp.pdf

mitochondria contigs • 1.8k views
ADD COMMENTlink modified 2.7 years ago by Biostar ♦♦ 20 • written 6.1 years ago by ckr12310
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