I'm a total noob... which tool would you recommend to use to design pimers against genes in a 'large' (800 Mbp) plant genome?
Hi Dan, we had similar questions quite often (too often to list all similar questions at least), when you just click on the primer tag you'll get ample help (or primer). So I think I should close it, sorry for that.
Sorry, I tried a search and only found discussions of specific cases of specific tools. I thought a specific question would be a good idea to focus the replies.
Yes, it would be a good idea, but what is specific about this question? Imho, your other question was more specific than this. Anyway, nothing much (afaik) has changed in state-of-the-art primer design.
I would also like to refer you to question from 3 years ago: How to find a 28bp 'primer' sequence in a genome? which has the most total votes under category 'primer', you might know it?
Thanks, I came here looking for that post actually, but couldn't find it. I guess I need to learn how to search better! I think it's a shame that this very specific question was closed, as I don't see any other question so clearly phrased. Please correct me if I'm wrong.
If you look at your user profile, you can see all your questions you have ever asked under: "N Questions" or see
http://www.biostars.org/show/user/105/question/ other content types respectively.
To me this is the quintessential example of why post closing is not useful.
All it adds a lot of back and forth arguments that is not about bioinformatics, adds to overall noise and negative sentiment.
If anything it decreases the utility of the site.
I think this is a special case where the user forgot about his own post and the post is just superficial and not helpful. So I see no reason to open it again.