Hi everyone, i am now analysis my Affymetrix porcine chip data, i have found different expression genes by fold change, here i want to ask for help that how to do GO analysis by bioconductor? would someone kindly tell me step by step, because i haven't learnt about R and Bioconductor, or if there is any papers describe this by detail? Thank you with advance.
I am not going to detail all the steps out, as they are described in the respective Bioconductor package vignettes. I will rather point you to the Bioconductor GO View page, where you will be able to find Bioconductor packages that deal with gene ontology.
If we take the
GOstats package as an example, there are three vignettes that describe all the ins and outs of how to perform your analysis.
Hope this helps!