Question: Negative Value On Linear Gene Expression In Microarrays
0
gravatar for fbrundu
5.6 years ago by
fbrundu280
European Union
fbrundu280 wrote:

Hi all, I am starting to use microarrays and maybe this is a dumb question:

Using Illumina microarrays, linear gene expression can be negative? Or maybe some artefacts have been introduced?

And, in this case, how to correct them? With scaling (adding the absolute value of the minimum negative value) or flooring (converting negative values to zeros)?

Thanks

ADD COMMENTlink modified 5.6 years ago by Sean Davis25k • written 5.6 years ago by fbrundu280
1
gravatar for Sean Davis
5.6 years ago by
Sean Davis25k
National Institutes of Health, Bethesda, MD
Sean Davis25k wrote:

This may result from background subtraction. To my knowledge, there is not a standard approach to dealing with this situation, but the two approaches you point out are not uncommon.

ADD COMMENTlink written 5.6 years ago by Sean Davis25k
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