Problem With Using Seqclean

Question

[meghasihag]

I am using seqclean for screening of EST sequences of cotton ball and fibre. I have installed all requirements such as perl, blast, database(Univec). But every time when I process any sequence in FASTA format in seqclean. It shows same result as starting with 'database is not indexed' and ending with 'no detectable error found'. I have done with approximate 100 sequences and got same result. Since emvec and trimest detect contamination in those sequences. Please tell me where I am doing it wrong.

[SES]

Can you check that you have legacy blast installed? That is what is required. If the vector files are not indexed, then an attempt to index them with formatdb is made, but if that program is not present then SeqClean will exit.