Comparison of ChIP-seq tracks from two cell lines using FC over control (ENCODE data)
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4 months ago
erica.fary • 0

Hi everyone,

I would like to compare H3K27ac ChIP-seq data at a specific genomic interval between 2 cell lines but I am a newbie in ChIP-seq... My plan is simply to proceed as follows:

  • download from ENCODE the rep1,2 bigWig files containing fold-change (FC) over control (1 file per cell line)
  • convert bigWig to bedGraph
  • boxplot of the values that fall within the genomic interval of interest (1 box per cell line)

My questions are the following:

  • is it correct to directly compare FC values across cell lines ? (my impression is that this provides a kind of normalization and makes the cell lines comparable, by I could not find a clear answer)
  • is it better to use rep1,2 file or to pool the rep1 and rep2 files ?
  • would it be better to compare cell lines using peaks BED data or signal values or signal p-values ? with what kind of normalization then to make data comparable across cell lines ?
  • or otherwise how would you have conducted this analysis ?

any help would be appreciated :)

Thanks

ChIP-seq H3K27ac bigWig ENCODE • 171 views
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