Entering edit mode
4.4 years ago
Joel
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0
Hellooo fellow B'informaticians, Well, I have a small learning stubble while using Artemis for multiple sequence alignment, there's a lot of documentation out there that guides you through using WebACT, doubleACT etc but I believe, the 2 mentioned online tools no longer exist? Could some gracious bioinfo buddy please help me understand the logic behind Multiple Genome comparison for 3 genomes? I mean how many comparison sequences do I need to have. How do I use the following code in that case:
makeblastdb -in genome1_fasta.fna -dbtype nucl
blastn -query genome2_fasta.fna -db genome1_fasta.fna -evalue 1 -task megablast -outfmt 6 > Genome1_Genome2.crunch
I don't know if I have framed the query in a comprehensive way enough but if anyone understood it please give your insights
