I used Enrichr for the functional enrichment of my gene list. I ranked the GO terms based on their combined score since it is a combination of p-value and z-score. My questions:
- Is combined score ranking better than p-value based ranking?
- Is 'infinity' combined score meaningful?
I referred to another post to answer these questions: the best combine score for enrichment analysis from Enrichr. This post provided guidance on the formula for a combined score(cs). Given that cs= log(p-value) * z-score, if p-value is very close to zero, then log value can go to -ve infinity. Otherwise, z-score should become infinity.
But from my result, it is clear that the p-value is not too low to make the combined score go to infinity.
Can anyone provide an insight on whether the infinity combined score is meaningful? The GO term associated with infinity score is extremely biologically relevant in our case, but we want to justify this. Thanks!!
