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2.9 years ago
Abdukhalim Khon
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0
Hello everyone. I have read some papers on Ka/Ks calculations. They said: first all the protein coding genes shold be extracted then Ka/Ks ratio may be calculated by Ka/Ks calculator.
I have 27 new sequenced chloroplast genomes and I think I should align these 27 species and extract all protein coding genes with Geneious software, export that extractions as a fasta format. Then calculate Ka/Ks ratios with Ka/Ks calculator.
But, how can I compare them, I mean should I compare that species one by one. Overal, more than 200 versions may appear. I think I am missing a step in the action, so I have a misunderstanding with it. This is my first independent attemp to do it.
Can anyone help me, please!
