Entering edit mode
3.3 years ago
gspirito
▴
10
Hello everyone, here's my question:
I have a dataset of RNA-seq data, where I have gene counts of different tissues from the same individuals in two conditions, like so:
In order to perform a differential expression analysis with DEseq2, comparing KO vs. WT of both tissues together, would it be correct to use this design?:
ddsFullCountTable <- DESeqDataSetFromMatrix(countData = counts.txt, colData = metadata.txt, design = ~Individual+Group, tidy=TRUE)
Thank you in advance