How to interpret scRNA-Seq cell dynamics from scVelo and CytoTrace results
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8 weeks ago
gundalav ▴ 360

I have the following scRNaseq analysis done with scVelo and CytoTrace. enter image description here

My understanding is as follows:

  1. scVelo arrows lead from less differentiated (more stem cell) to more differentiated (e.g. granulocyte)
  2. CytoTrace brighter color is more differentiated (e.g. granulocyte) to darker color less differentiated (e.g. more esteem)

If so, the two results I have above are totally opposite. Is my interpretation correct? If so, why did it give the opposite result? Which one should we trust: scVelo or CytoTrace?

velocyto cell cytotrace rnaseq dynamics • 130 views

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