Creating Signature Matrix from SC-RNA Seq data for Deconvolution of Bulk Tissue
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3 months ago

Hey,

I've been trying to understand how to create a signature matrix but there's no tutorials to follow and the papers I've found from Cibersort and others lack in detailing step by step. So, can anyone provide some insights on the matter. One paper that I have followed intimately has this section (image attached below) but the resulting matrix doesn't work in downstream processing.

enter image description here

deconvolution rna-seq cibersort • 299 views
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Hello, Cibersortx website has a nice tutorial about it. To clarify, I suggest you to go on this thread I posted few months ago to know more : [SOLVED] How to build a table with gene expression per cell type with Seurat ?

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