Deleted:Where is the annotation file if using the GtRNAdb (tRNA SE analysis) for mapping to RNAseq libraries?
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2.6 years ago

Hi all,

On the GtRNAdb (tRNA-SE analysis) website there is a file containing fasta sequences of different tRNA genes. http://gtrnadb.ucsc.edu/genomes/eukaryota/Hsapi38/

I aligned this GtRNAdb database with RNAseq libraries using bowtie2 and got a fair amount of mapped reads. However now the problem is that there is no .gtf or .gff3 available to my knowledge to annotate the extracted reads mapped to tRNA. The read identifier name in the fasta file does contain information about the tRNA subtype which could potentially be used for annotation.

My question: How to proceed with the tRNA annotation? Should I try to generate my own annotation file?

Thanks!

kind regards,

Patrick

GtRNAdb tRNA-SE tRNA analysis annotation RNAseq • 534 views
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