Entering edit mode
2.6 years ago
Info.shi
▴
30
Dear all,
I have question I mapped reads on cds sequence through STAR I don't have gtf file and want to calculate read count using RSEM but I am stuck by error "RSEM error: RSEM currently does not support gapped alignments" as I don't have intron so I can not define.
Mapping command: STAR --genomeDir ref/ --runThreadN 4 --readFilesIn R1.fq.gz R2.fq.gz --readFilesCommand zcat --outFilterMismatchNmax 2 --outFileNamePrefix
If anybody suggest me regarding this I will be very thankful.
