Question

bowtie2 and then HISAT2? What does it mean in Data processing details on a paper?

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2.5 years ago

Simon Ahn ▴ 10

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This is an data processing detail from https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM3056968. \ It says they used bowtie2, which is an alignment tool, and then used TopHat (now HISAT2), which is also an alignment tool. \ I have no idea why they aligned their reads twice. Is this just a mistake? or is there something that I don't know?

RNAseq fastq raw-count • 947 views

ADD COMMENT • link updated 2.5 years ago by Chris Dean ▴ 390 • written 2.5 years ago by Simon Ahn ▴ 10

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It was probably an oversight. It looks like the data was made publicly available two years prior to the publication. A lot of things can change during that time. Have a look at the methods described in their published paper and see if that clears things up (see Section 4.2).

ADD REPLY • link 2.5 years ago by Chris Dean ▴ 390

score 2 · Accepted Answer · 2021-10-20

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2.5 years ago

Philipp Bayer 8.3k

IMHO that must be a mistake: the data seems to be RNA so I would've directly gone for TopHat2 (or HISAT2).

(Unless they wanted to run Fastqc in BAM, not fastq mode - in which case Tophat2's bam would've worked too?)

ADD COMMENT • link 2.5 years ago by Philipp Bayer 8.3k